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The Complete COVID-19 Extraction and Detection Kit (spin column)

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Size: 50 Rxns
DG: No
Storage: 2 to 8°C; -15 to -20°C*
Sterile: No
Stock: In stock

Product Description:

The Complete COVID‐19 Extraction and Detection Kit allows an All‐In‐One solution for the Extraction of Viral RNA, followed by its detection via RT‐PCR.

This complete kit comprises of the following two kits:
I. COVID-19 Viral RNA Extraction Kit (Cat # VT9200 )
II. SARS‐CoV‐2 RT‐PCR Detection Kit (Cat # CoV‐2‐RTPCR)

I. COVID-19 Viral RNA Extraction Kit (Cat # VT9200 )

The kit is especially designed for COVID-19 (SARS-CoV-2). It simplifies the isolation of COVID-19 viral RNA from cell-free body fluids with a fast spin-column format. No phenol/chloroform extraction is required. Viral RNA binds specifically to the silica membrane while contaminants are removed in the flow-through. PCR inhibitors such as divalent cations and proteins are completely removed in two efficient wash steps, leaving pure viral RNA to be eluted in RNase-free water. Purified RNA is ready to use in RT-PCR, Northern blotting, or other downstream applications.

Features:

► Fast: Using a rapid spin-column format, the entire procedure takes about 20 minutes.
► High Yield: The recovery yield of viral RNA is generally >85%.
► Versatile: Suitable for purification of viral RNA from Nasopharyngeal swabs (dry) or Transport medium/Viral transport medium (VTM) containing Nasopharyngeal swabs, Nasopharyngeal aspirates, and bronchoalveolar lavage (BAL) samples.
► Non-toxic: No phenol/chloroform is used.

*Storage:
Carrier RNA should be divided into conveniently sized aliquots and stored at –20°C. Do not freeze-thaw the aliquots of Carrier RNA more than 3 times. All remaining components should be stored at room temperature. The kit is valid for 1 year at 4°C.

II. SARS‐CoV‐2 RT‐PCR Detection Kit (Cat # CoV‐2‐RTPCR )

Our newly developed RT-PCR kit can be used on human respiratory tract specimens and serves as an ideal molecular diagnostic tool to detect COVID-19 / SARS-CoV-2. In order to assess the kit’s performance, it has been clinically tested and approved by the National Centre for Clinical Laboratories (NCCL) in Beijing.

Features:
► Highly specific for the ORF1ab and N target markers recommended by the World Health Organization (WHO) and the Centers for Disease Control and Prevention (CDC) in the United States.
► Ready-to-use reaction kit that includes MasterMix, Primers/Probes, and Internal Positive Control.
► Can be used with samples collected from sputum and naso/ oropharyngeal swabs.
► Compatible with standard RT-qPCR machines (Applied Biosystems 7500, QuantStudio 7 Flex, or Bio-Rad CFX96 recommended).
► Clinically tested and approved by the National Centre for Clinical Laboratories (NCCL).

SARS-CoV-2 RT-PCR Detection Kit is a one-step RT-PCR Kit for the detection of SARS-CoV2-specific RNA in specimens including serum from suspected patients. The kit contains SARS-CoV-2-specific primer and probe sets designed by comparing all available sequences of other coronaviruses that allows both transcription and subsequent PCR amplification in a single reaction. An internal control for human RNA species will be provided with the kit to identify possible PCR inhibition and assess the reliability of the PCR reaction.

The limit of detection for this kit is 1×10^3 copies (Ct±37)

This Assay is validated on the Applied Biosystems® 7500 Fast and 7500 Real-Time PCR system.

*Storage:
‐10°C to ‐30°C for all components.

Total Product Size:
50 Rxns

Kit Test Results and Analysis (473.37 KB)

RT-PCR Setup: ABI 7500 (1.09 MB)

COA – RNA Extraction (865.08 KB)

COA – RT-PCR Detection – 1 (409.74 KB)

COA – RT-PCR Detection (example) (56.96 KB)

SDS – RT-PCR Detection (410.89 KB)

Technical Inquries:

For product support/troubleshooting,
please email us your inquiry at:
sales@biotrackdiagnostics.com

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Contact us

Bio Track Diagnostics
440 - 1040 W Georgia St, Vancouver, BC

 1 587-999-9669

sales@biotrackdiagnostics.com